Intracellular Cytokine Staining

bernie bellette at
Tue Sep 5 21:11:10 EST 2000

Work in my department involves determining the intracellular cytokine
stores of murine CD4 and CD8 cells.  Following staining and
assessment via flow, results to date have not demonstrated many
positive  cells, even in cell lines known to be producers of the two
cytokines we are assaying for, IL-4 and IFN-gamma.  Would anyone have
any trouble shooting tips or know of any cell lines that are
producers of these cytokines that come pre-stained for intracellular
cytokines that we can use as a positive control.

Many thanks
Bernadette Bellette
PhD Student
Discipline of Pathology
University of Tasmania

(03) 6226 4762 (phone)
(03) 6226 4833 (fax)

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