Leukemia Flowcytometry - Threshold for positivity

Bakul Dalal (Dr.) bdalal at vanhosp.bc.ca
Fri Sep 1 16:55:50 EST 2000


The FS/SS primary gating technic tended to include non-blast cells (mainly lymphocytes)
within it. That was one of the reasons the 20% threshold was applied to conclude a blast
"positive".

However, the CD45/SS gating excludes the lymphocytes. There may be a small number of
non-leukemic blasts in the gate, but the rest are all leukemic blasts.

For those using CD45/SS gate, what is your threshold for positivity?

Many thanks...

Bakul I. Dalal, MD FRCPC FCAP FASCP
Associate Director, Hematology Laboratory
Director, Flow Cytometry Laboratory
Vancouver, General Hospital
Clinical Professor, Faculty of Medicine
University of British Columbia
910, West 10th Avenue
Vancouver, BC, Canada, V5Z 4M3
604 875 4496/4633(f)/877 5949(pgr)




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