isotype question

Maciej Simm simmmmer at yahoo.com
Fri Oct 13 13:45:16 EST 2000


Hello all,

I wonder if mixing 2 antibodies with the same isotype is a good idea?
IE. can I have a mouse antihuman IgG1 antibodies with different
specificities/color conjugates in the same tube? I wonder what the
11-color flow experts have to say about that :)

part 2,
are there special compensation precautions for mixing BD's PE with
Caltag's TC antibodies? it seems both channels are amplified a bit as
opposed to being run separately..

Maciej

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