anti-mouse surface IgG

Jennifer Bell jen.bell at ed.ac.uk
Sun Mar 12 10:20:16 EST 2000


Can anyone recommend a good antibody against mouse IgG to be used as a
primary antibody on murine B cells?

So for I've tried FITC conjugated polyclonals against IgG and IgG1 from
Southern Biotech and Sigma respectively and home grown mMb 20.9  (anti-IgG1
a allotype).  The polyclonals seem to cross react with sIgM/D.  The
monoclonal while working well in ELISA failed to stain at all  by FACS. The
polyclonals also cross reacted strongly with rat mAbs which is a problem as
I want to stain for multiple surface markers.

Any suggestions much appreciated.

Jennifer

______________

jen.bell at ed.ac.uk
Institute of Cell, Animal and Population Biology
University of Edinburgh
Kings Buildings
Edinburgh EH9 3JT
UK

tel.  0131 650 5556
fax. 0131 650 7322





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