anti-mouse surface IgG

Jennifer Bell jen.bell at
Sun Mar 12 10:20:16 EST 2000

Can anyone recommend a good antibody against mouse IgG to be used as a
primary antibody on murine B cells?

So for I've tried FITC conjugated polyclonals against IgG and IgG1 from
Southern Biotech and Sigma respectively and home grown mMb 20.9  (anti-IgG1
a allotype).  The polyclonals seem to cross react with sIgM/D.  The
monoclonal while working well in ELISA failed to stain at all  by FACS. The
polyclonals also cross reacted strongly with rat mAbs which is a problem as
I want to stain for multiple surface markers.

Any suggestions much appreciated.



jen.bell at
Institute of Cell, Animal and Population Biology
University of Edinburgh
Kings Buildings
Edinburgh EH9 3JT

tel.  0131 650 5556
fax. 0131 650 7322

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