Isolation of kupffer cells

Lee Szu Hee shlee at
Fri Jun 30 03:24:28 EST 2000

Hi Colin

We isolated mouse Kupffer cells by collagenase perfusion-digestion in this

Lee SH, Crocker PR, Gordon S. Macrophage plasma membrane and secretory
properties in murine malaria. J Exp Med. 163:54-74. 1986.

Best regards
Szu-Hee Lee
Dept of Pathology
National University of Singapore

-----Original Message-----
From: Tom Mc Closkey [mailto:thomasm at]
Sent: Thursday, June 29, 2000 3:31 AM
To: cyto-inbox
Subject: Re: Isolation of kupffer cells

arturo Colin Martinez wrote:
> I´m looking for information on how isolate kupffer cells from mouse´s
> and spleen. If any one can direct me to appropriate references and/or has
> any practical suggestions, I would greatly appreciate hearing from you.

	You might find the following reference useful:

Mc Closkey, TW, et al.	Lipopolysaccharide treatment of rats alters
antigen expression and oxidative metabolism in hepatic macrophages and
endothelial cells, Hepatology, 16: 191-203, 1992.

This work was done in a rat model system but describes Kupffer cell
isolation and the method should be similar.

Good luck,

Thomas W. Mc Closkey, Ph. D.
Director of Flow Cytometry, North Shore University Hospital
Assistant Professor of Pediatrics, New York University School of
Boas Marks Biomedical Research Center, 350 Community Drive
Manhasset, Long Island, New York 11030
ph:  516-562-4844 [office], 516-562-1135/4641 [lab]  fax:  516-562-2866

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