fixed sample sorting

Andreas Simm simm at klin-biochem.uni-wuerzburg.de
Thu Feb 10 02:15:19 EST 2000


We tried to isolate RNA from fixed samples
and had bad luck with formaldehyd fixed 
samples.
So, we used ethanol fixed cells for sorting
and RNA preparation.

We have published the method in:

Diez C, Bertsch G und Simm A
Journal of Biochemical and Biophysical Methods, 40 (1999) 69-80
“Isolation of Full-size mRNA from Cells Sorted by Flow Cytometry”

Diez C und Simm A
Cardiovascular Research, 40 (1998) 530-537
“Gene Expression in rod shaped cardiac myocytes, sorted by flow 
cytometry”

There is another paper from the group of Radbruch, using a 
combination of ethanol and acetic acid for fixation:
Esser et al., Cytometry 21 (1995) 382-86

Hope this helps

Andreas




My address:

PD Dr. Andreas Simm
Institut fuer Klinische Biochemie und Pathobiochemie
Versbacher Str. 5
D-97078 Wuerzburg
Germany

Tel.:   +49 931-201-3144
FAX.: +49 931-201-3153
E-mail: simm at klin-biochem.uni-wuerzburg.de



More information about the Cytometry mailing list