Antibody Binding Capacity - Autofluorescence
Kara E. McCloskey
mccloskey at che.eng.ohio-state.edu
Tue Feb 8 16:56:52 EST 2000
I am trying to calculate ABC for cells with very high levels of autofluore-
scence. Two questions:
1) Is it really accurate to simply subtract off the ABC for unstained cells?
2) Is it OK to extrapolate outside of the calibration plot for ABC vs.
Thanks in advance,
Graduate Student, OSU
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