Antibody Binding Capacity - Autofluorescence

Kara E. McCloskey mccloskey at
Tue Feb 8 16:56:52 EST 2000

Hi all,

I am trying to calculate ABC for cells with very high levels of autofluore-
scence.  Two questions:

1) Is it really accurate to simply subtract off the ABC for unstained cells?

2)  Is it OK to extrapolate outside of the calibration plot for ABC vs. 
channel number?

Thanks in advance,

Kara McCloskey
Graduate Student, OSU

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