Stimulating monocytes

Hodge, Greg (HAEM) hodgeg at mail.wch.sa.gov.au
Mon Aug 21 00:31:30 EST 2000




> ----------
> From:		Vivian Wu
> Sent:		Friday, 18 August 2000 13:22
> To:	Cytometry Mailing List
> Subject:	Stimulating monocytes
>
> Vivian,
>
>   I have noticed increased loss of CD14 and CD33 on monocytes with
> increasing concentration of LPS used.
> I have success stimulating i/c cytokines with 100 ng/ml LPS in a whole
> blood 24 h assay and still have appreciable CD14 and CD33 staining.
>
> Regards,
> Greg Hodge PhD
>	Flow Lab/ Haematology Department,
>	Women's and Children's Hospital
>	North Adelaide,
>	South Australia  5006
>	ph: 08 82046522
>	fax: 08 82046043
>	email: hodgeg at mail.wch.sa.gov.au
> --------------------------------------------------------------------------
> --------------------------
>
> On 20/8/00 Vivian wrote:
> Hi All,
>
> I have been stimulating monocytes with LPS and adding monensin into the
> culture for the last 4 hours. The CD14 and CD33 antigens were down
> regulated
> after 24 hours of stimulation. I would like to be able to gate on
> monocytic
> antigens while doing cytokine analysis. Are there other monocytic antigens
> which will not be down regulated after 24 hours of stimulation?  I'd
> appreciate any suggestions and thanks in advance.
>
> Vivian Wu
> Immunology Lab.
> Vancouver General Hospital
> B.C.
> email: ffwu at interchange.ubc.ca
>



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