annexin V dim cells
shawn_jackson at student.hms.harvard.edu
Wed Apr 12 09:19:20 EST 2000
I have a question concerning the occurrence of annexin V-dim cells vs. bright
cells. I need help with references and/or data experience!
I am staining peripheral blood from mice infected with vaccinia, and then
staining the CD8 cells with annexin V-fitc. I see a population of annexin+
cells extending even up past the 2nd log at many time points, but at certain
times I see very bright cells up at the 3rd log.
I have been warned that only these very bright cells are truly AV+, cells with
any intentions whatsoever of doing anything related to a death process, while
the dimmer cells represent actively proliferating cells only, with no
intentions of dying. and these dimmer cells are likely to return to an AV-
Could anyone help me with references or personal experince addressing the
status of these AV dim cells in contrast to bright ones?
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