Dentritic Cell Markers

Van Bockstaele, Dirk Dirk.Van.Bockstaele at uza.uia.ac.be
Fri Oct 29 03:20:55 EST 1999


Dear Meral,

In my opinion, the best way to obtain dendritic cells is to cultivate them
out of BM progenitors that are easy to isolate (CD34+ ).  The culture in
GM-CSF and TNF-a (and maybe some additional cytokines) results in an
abundant amount of DC after 2 weeks.
You may want to read relevant literature: group of Caux and Bancherau is a
good starting point (and endpoint!)
We have a modest contribution in:
Generation of DC from BM progenitors using gm-csf, tnf-a, and additional
cytokines: antagonistic effects of il-4 and ifn-g and selective involvement
of tnf-a receptor-1.
Lardon et al. Immunology 1997, 91: 553-559

Off course all this is on human DC but I think it also holds for mice work.
Best Regards,
Dirk

Prof. Dirk Van Bockstaele, PhD
Laboratory of Hematology
Head Flow Cytometry
Antwerp University Hospital 
Belgium
phone 32 3 821 3900, fax 32 3 825 1148


> ----------
> Van: 	keskinte at immagene.mcg.edu[SMTP:keskinte at immagene.mcg.edu]
> Verzonden: 	woensdag 27 oktober 1999 19:41
> Aan: 	Cytometry Mailing List
> Onderwerp: 	Dentritic Cell Markers
> 
> 
> Dear Group,
> 
> I have a user that needs to isolate dentritic cells from mice. We would
> like to hear about new dendritic cell markers. Any experience and
> suggestions would be appreciated. Thanks in advance.
> 
> --------------------------------------------------------------------------
> ----
> 
> Meral Keskintepe, PhD.
> 
> Institute of Molecular Medicine and Genetics
> Medical College of Georgia
> 15th street CA-2022
> Augusta, GA. 30912-2600
> 
> Tel: (706) 721-8880
> Fax: (706) 721-8732
> 
> --------------------------------------------------------------------------
> --
> --
> 
> 



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