Hepatocyte Isolation Protocol

Adamson,Lee LAdamson at shrinenet.org
Thu Oct 14 10:44:44 EST 1999


I am looking for a protocol for the isolation of mouse hepatocytes for
immediate use on flow.  I have found several old protocols isolating rat
hepatocytes, but the majority of these entail growing the hepatocytes in
culture or fixing in ethanol for future use.  I am looking to analyze by
flow immediately with Annexin V and PI and none of these protocols seem to
be compatible.  Thanks for any input.

Lee Adamson
Shriners Hospital for Children Northern California



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