Sheath Tank Filling

Carol Oxford cloxford at UCDAVIS.EDU
Fri Oct 8 15:53:04 EST 1999



Wow!  I'm starting to feel a little inadequate here, but you guys are
working a lot harder than I am.  I have a .2 um in-line filter on my MoFlo,
so I rinse the tank with a little ethanol at the beginning of the week,
then fill up the tank daily in the open.  We've never had a contamination
yet, and we routinely swich back and forth between bacteria, yeast, and
mammalian cells.  I pay a lot more attention to the sample lines- I use
ethanol, CoulterCleanse, and sterile H20 to set up for a sterile sort, but
I don't ever pay much attention to the sheath tank.

Carol

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Carol Oxford                             _|    _|   _|_|_|_|   _|_|_|
Manager, UC Davis Optical Biology Lab   _|    _|   _|         _|    _|
Tupper Hall, rm 3439		       _|    _|	  _|         _|    _|
University of California              _|    _|   _|         _|    _|
Davis, California  95616             _|    _|   _|         _|    _|
(530) 752-7205                      _|    _|   _|         _|    _|
(530) 752-4548 fax                 _|_|_|_|   _|_|_|_|   _|_|_|
(530) 523-0967 pager
cloxford at ucdavis.edu               University of California, Davis
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