Ann Atzberger Ann.Atzberger at EMBL-Heidelberg.de
Fri Nov 26 09:20:39 EST 1999

Hi Carl,

I use FACSFlow for just about everything, no problem as long as users
realise they have to centrifuge their samples after sorting. It doesn't
seem to have any adverse effects when cell cloning into 96well plates. I
suspect that there's more than just azide in this sheath fluid as literally
nothing will grow in it. It probably contains a strong growth inhibitor and
it may be this that is causing the problem with the Terasaki plates. The
dilution of the medium by the sheath fluid being too high. I would suggest
doing a one-drop sort if you are not already doing so and using a nozzle as
small as possible to get smaller drops.

good luck
Ann Atzberger

At 11:02 24.11.99 +0100, you wrote:

>Dear Friends,
>For both sorting and analysis we use sheath fluid sold under the name "FACS
>Flow" by BD (in Europe). This, I've been told, is basically PBS with an
>azide component as preservative. Generally this works fine but when it comes
>to single cell sorts we tend to believe that the small amount of FACS Flow
>that falls into the 20µl Terasaki well has detrimental effects on the cell.
>What are your experiences with this reagent? What are you using and from
>what suppliers? Has the azide component any positive/negative effects on the
>analyzed material or is it simply there as an antimicrobial agent?
>Thank you for your answers.
>Carl-Magnus Högerkorp
>Stem Cell Laboratory
>University Hospital of Lund

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