CK Abs

Valery Kotelnikov VKotelnikov at
Fri Sep 25 12:11:21 EST 1998

I am titrating cytokeratin antibodies directly conjugated to Cy3 or PE.
The problem is: using cultured tumor cells I can reach the plateau at
only 15 ug/ml which seems too high. I am using 0.05 saponin for
permeabilization.  Does anybody have any experience in
this field?  I tried CAM5.2, C11, AE1, LP34 and pan CK (a mixture of
different Abs).

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