re (n): RNA from sorted cells
jdunne at BDIS.Com
Mon Nov 2 20:05:40 EST 1998
thanks for the memories..
yes we published on RNA from sorted cells (Cytometry 10:199-204,1989),
but it was just spot-blots, not northerns, no isolation and
electrophoresis, so no transcript size info. The cells were sorted
live onto nitrocellulose and mRNA stuck onto the filter with a
commercial prep (S+S "Quick-blot'). We saw good specificity and
linearity for IL1b message using U937 cells induced with PMA+LPS.
jdunne at bdis.com
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