re (n): RNA from sorted cells

John Dunne jdunne at BDIS.Com
Mon Nov 2 20:05:40 EST 1998


     thanks for the memories..
     
     yes we published on RNA from sorted cells (Cytometry 10:199-204,1989), 
     but it was just spot-blots, not northerns, no isolation and 
     electrophoresis, so no transcript size info.  The cells were sorted 
     live onto nitrocellulose and mRNA stuck onto the filter with a 
     commercial prep (S+S "Quick-blot').  We saw good specificity and 
     linearity for IL1b message using U937 cells induced with PMA+LPS.
     
     still happy,
      Jack Dunne
     jdunne at bdis.com



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