dim CD4 population

Barbara Butcher bab26 at cornell.edu
Thu Mar 19 12:08:21 EST 1998

hi there--

a colleague biotinylated anti-horse CD4 antibodies and now sees a dim
population  with streptavidin-FITC which was not there when she used an
anti-IgG FITC as secondary. (this is in addition to the "standard"
population). is there a technical reason for this, or is this a real,
heretofore unseen population?  does it have something to do with a subset
of antibodies that weren't biotinylated as efficiently?  oh--these cells
look like they're NK+.

thanks for your replies!


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