drbal at leuko.biomed.cas.cz
Mon Jun 29 04:25:08 EST 1998
Try to strip the fluorochrome labelled Ab using ice-cold acid isotonic
citrate/phosphate buffer (pH 3.0) (0.263M citric acid/0.123M HPO4 +
protein) for a very short time (10 seconds - 1 minute) and neutralize the
cell suspension immediately. Spin down the cells, aspirate all supernatant
and wash once more and restain with another Ab. This treatment should not
harm your sorted cells. Or do you want to strip just the fluorochrome from
the bound Ab?
Hope this helps.
Ken McDonald wrote:
> Hi Flowers...I had a question from a Facs user who wants to know if
> after sorting a cell population with let's say a FITC stained antibody,
> is there a way of then stripping that fluorocrome and re-staining with
> another FITC stained antibody, all the while leaving the cell viable?
> She needs this to happen right after sorting.
> Thankyou in advance...Ken
> -- End --
Laboratory of Leukocyte Antigens
Institute of Molecular Genetics
Academy of Sciences of the Czech Republic
142 20 PRAHA 4
Czech Republic, Europe
e-mail: drbal at leuko.biomed.cas.cz
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