How can you reduce macrophage autofluorescence?

Dr. Brian D. Robertson b.robertson at
Wed Feb 25 12:22:26 EST 1998

Would anyone be able to supply a method for reducing macrophage
autofluorescence on flow cytometry with GFP (FITC) bacteria ? The
background fluorescence seems stronger than my signal. Can Trypan blue or
ethidium bromide be used with good results ?

Thank you very much for your replies,
Val Snewin
vas31 at

Dr. Brian D. Robertson,
Medical Microbiology,
Imperial College School of Medicine,
St. Mary's Campus,
Norfolk Place, London W2 1PG, U.K.

Fax: 0171-262-6299   email: b.robertson at

Futura formare prospicere melius.
(It is better to shape the future than to try and forsee it)

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