low cell count specimen

Vincent Gin ssni at ix.netcom.com
Wed Feb 25 10:28:09 EST 1998

Can anyone shed some light on how they handle specimen that has very 
low cell count e.g. <2500/mm3, CSF, FNA etc. Do you dilute antibody 
concentration or cut back amount of antibody to accomodate the same 
ratio of cell:antibody? We sometimes get specimen that has cell count 
of <500/mm3 and flow was preferred than IP slide method. Thanks in 
advance. Nancy Gin - United Hospital, St.Paul, MN 

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