sort viability

Echeagaray, Patricia L. echeagaray at sri.org
Thu Aug 27 11:37:02 EST 1998


Does the media in which they sit prior to sorting, as well as the media
they are sorted into, contain any protein? (BSA or FBS)

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Patricia L. Echeagaray;  echeagaray at sri.org
Flow Cytometry Services
Southern Research Institute - Frederick
Pharmaceutical Research and Development  Dept.
431 Aviation Way     
Frederick, Maryland 21701
USA
Voice: (301) 228-2170 or (301) 694-3232 ext 111 or 276
   FAX: (301) 694-7223
echeagaray at sri.org
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>----------
>From: 	Mark Cozens[SMTP:cozens at postoffice.utas.edu.au]
>Sent: 	Sunday, August 23, 1998 9:06 PM
>To: 	Cytometry Mailing List
>Subject: 	sort viability
>
>
>Dear flowers,
>	Thanks for all the helpful responses so far concerning my problem
>with obtaining viable sorts. Perhaps this additional information may be of
>use.
>The cells I am sorting are murine splenic lymphocytes. Directly post sort
>their viability is 80% (tyrpan blue).
>Flow details: Coulter Elite ESP sorter, power 15mW air cooled argon, 100um
>nozzle, sheath straight PBS, sort speed @3000/sec, pressure @11 psi
>
>I've done several test sorts and my results indicate an increase in
>viability with the laser off (collecting everything) although viability
>still remains low. Is there a possibility that laser power could be
>affecting my cells in some way?
>
>The concerned student, James.
>
>
>Mark A Cozens
>Division of Pathology
>University of Tasmania
>ph	03 62264828
>fax	03 62264833
>
>
>
>
>



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