Trypsin and Annexin
Dr. Szollosi Janos
szollo at jaguar.dote.hu
Tue Aug 25 02:29:18 EST 1998
In our hand trypsin caused only a 5-10 % decrease in the expression level
of erbB2 protein, suggesting that not all cell surface proteins are
stripped of during trypsin treatment.
We compared trypsinized cells with EGTA harvested ones.
I think that the stripping effect depends upon the cell lines and the
receptors you investigate.
At 03:11 PM 8/19/1998 -0500, you wrote:
>Well, Trypsin has the reputation of stripping cell surface markers, and
>can't it kill off cells if the concentration is strong enough? Also, if
>cells surface markers are stripped and cells are starting to die, or are
>damaged somehow, they can exert autoflourescense.....or so I have found
>with cultured cells. Sorry, no exp. with Annexin, but I will soon as my
>kit arrived yesterday!
>Patricia L. Echeagaray; echeagaray at sri.org
>Manager of Flow Cytometry Services
>Southern Research Institute - Frederick
>Pharmaceutical Research and Development Dept.
>431 Aviation Way
>Frederick, Maryland 21701
>Voice: (301) 228-2170 or (301) 694-3232 ext 111 or 276
> FAX: (301) 694-7223
> echeagaray at sri.org
>>From: Joan Kalnitsky[SMTP:jkalnits at mail.vt.edu]
>>Sent: Tuesday, August 18, 1998 10:11 AM
>>To: Cytometry Mailing List
>>Subject: Trypsin and Annexin
>>I have a client who is staining with Annexin-FITC. They are looking at
>>cell lines and are using trypsin to get the cells off the flasks. We are
>>seeing what we think is an exagerated Annexin response. Does anyone out
>>there have any feedback on how trypsin affects Annexin staining? Any
>>experience and or information about this will be greatly appreciated.
>> Thanks in advance,
>> Joan Kalnitsky
>>Flow Cytometry Laboratory
>> "It is better to serve than to receive"
>> B. Borg
More information about the Cytometry