Graca Almeida-Porada almei_g at med.unr.edu
Mon Aug 24 13:14:37 EST 1998

I had the same problem.  I was using FACSflow a couple years ago and the
viability of the cells were fine and suddenly all my cells were dead
after sorting What I was told by a BD representative was that they had
changed the composition of the sheeth fluid and it was no longer
suitable for live sorting. Since then I've been using PBS with 1%
PenStrep 0.1% Fungizone with good results.

Graça Almeida-Porada, M.D., Ph.D

University of Nevada School of Medicine
Department of Medicine
VA Medical Center
1000 Locust St
Reno NV 89520

Phone 702-328 1232
Fax     702-328-1745

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