Newsom, Brian S. bsnewsom at msmail.his.tch.tmc.edu
Mon Aug 24 13:40:00 EST 1998

We found the same thing. BD does not advertise that their sheath is   
toxic, but indeed it is. If you ask them they usually quote the line you   
gave. We tested the sheath on cells without sorting and it killed them   
within 15 minutes of incubation. We make our own sheath now and we have   
no problems.

Brian Newsom
Center for Cell and Gene Therapy
Baylor College of Medicine

From:  Bill Throndset
Sent:  Monday, August 24, 1998 11:53 AM
To: cyto-inbox
Subject:  FACSflow

I recently had to switch from Criterion Diluent 2, Azide Free to   
Lo and behold, all of my cells died.

I spoke to a BDIS representative and she told me that FACSflow "can be a
problem for live cell sorting."

Needless to say, I was extremely unhappy that there was no indication of
this in the product description in the BD catalog, or on the boxes of
diluent themselves.

Has anyone else found this to be the case?

Since then, I have used PBS, Coulter Isoflow, and now I'm back to
Criterion, with no viability problems.

Perhaps others have have learned the hard way as well?


bill throndset
bthrondset at rigelinc.com
Rigel, Incorporated

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