cozens at postoffice.utas.edu.au
Sun Aug 23 20:06:17 EST 1998
Thanks for all the helpful responses so far concerning my problem
with obtaining viable sorts. Perhaps this additional information may be of
The cells I am sorting are murine splenic lymphocytes. Directly post sort
their viability is 80% (tyrpan blue).
Flow details: Coulter Elite ESP sorter, power 15mW air cooled argon, 100um
nozzle, sheath straight PBS, sort speed @3000/sec, pressure @11 psi
I've done several test sorts and my results indicate an increase in
viability with the laser off (collecting everything) although viability
still remains low. Is there a possibility that laser power could be
affecting my cells in some way?
The concerned student, James.
Mark A Cozens
Division of Pathology
University of Tasmania
ph 03 62264828
fax 03 62264833
More information about the Cytometry