Trypsin and Annexin

Echeagaray, Patricia L. echeagaray at
Wed Aug 19 15:11:03 EST 1998

Well, Trypsin has the reputation of stripping cell surface markers, and
can't it kill off cells if the concentration is strong enough? Also, if
cells surface markers are stripped and cells are starting to die, or are
damaged somehow, they can exert autoflourescense.....or so I have found
with cultured cells. Sorry, no exp. with Annexin, but I will soon as my
kit arrived yesterday! 


Patricia L. Echeagaray;  echeagaray at
Manager of Flow Cytometry Services
Southern Research Institute - Frederick
Pharmaceutical Research and Development  Dept.
431 Aviation Way     
Frederick, Maryland 21701
Voice: (301) 228-2170 or (301) 694-3232 ext 111 or 276
   FAX: (301) 694-7223
    echeagaray at

>From: 	Joan Kalnitsky[SMTP:jkalnits at]
>Sent: 	Tuesday, August 18, 1998 10:11 AM
>To: 	Cytometry Mailing List
>Subject: 	Trypsin and Annexin
>I have a client who is staining with Annexin-FITC.  They are looking at
>cell lines and are using trypsin to get the cells off the flasks.  We are
>seeing what we think is an exagerated Annexin response.  Does anyone out
>there have any feedback on how trypsin affects Annexin staining?  Any
>experience and or information about this will be greatly appreciated.
>	Thanks in advance,
>	Joan Kalnitsky
>Joan Kalnitsky
>Flow Cytometry Laboratory
>(540) 231-4115
>FAX 540-231-7367
>	"It is better to serve than to receive"
>	 B. Borg

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