Trypsin and Annexin

Joan Kalnitsky jkalnits at mail.vt.edu
Tue Aug 18 14:11:23 EST 1998


I have a client who is staining with Annexin-FITC.  They are looking at
cell lines and are using trypsin to get the cells off the flasks.  We are
seeing what we think is an exagerated Annexin response.  Does anyone out
there have any feedback on how trypsin affects Annexin staining?  Any
experience and or information about this will be greatly appreciated.
	Thanks in advance,
	Joan Kalnitsky
Joan Kalnitsky
Flow Cytometry Laboratory
VMRCVM
(540) 231-4115
FAX 540-231-7367

	"It is better to serve than to receive"
	 B. Borg


	




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