Sterilizing Sorter

Nicholson, Janet jkn1 at cdc.gov
Fri Aug 14 14:04:00 EST 1998


The reference is Cytometry 28:99-117 (1997).  The table Steve refers to
is adapted from one at Lawrence Livermore National Laboratory.

> -----Original Message-----
> From:	Steve Merlin [SMTP:merlin at aitken.org]
> Sent:	Thursday, August 13, 1998 7:24 PM
> To:	Cytometry Mailing List
> Subject:	RE: Sterilizing Sorter
> 
> Dennis, 
> 
> For killing vegetative spores in the fluidics, use 10% household
> bleach or 3-6% hydrogen peroxide. 70% ethanol will not kill resistant
> vegetative spores. I 'm not sure about acetic acid being totally
> effective. Ingrid Schmid of UCLA was an author an a biosafety article
> in Cytometry published last year with a table listing various agents
> agents, there recommended uses and effectiveness. I don't have the
> citation at hand--Ingrid or anyone that has the issue and page numbers
> handy of this article to add to the posting?
> 
> Steve
> 
> Steven Merlin
> Aitken Neuroscience Center
> Flow Cytometry Facility
> 523 East 72nd Street/8th Floor
> New York, N.Y. 10021
> 212-772-6426 (Lab)
> 212-772-0608 (Office)
> 212-772-0357 (Fax)
> merlin at aitken.org
> 
> Dennis J. Young wrote:
> >
> >Currently I sanitize the in line filter (and everything down stream)
> with
> >sequential 60 ml 10% acetic acid, followed by 60 ml 70% ethanol (both
> in
> >water). I let them "soak in" for 10 minutes. This is then followed by
> PBS.
> >
> >Could I save time by using 10% acetic acid IN 70% ethanol (one
> solution,
> >one soak) and still kill every living thing in the sheath lines? 
> >
> >
> >---> Dennis
> >
> >*****************************************************************
> >********
> >Dennis J. Young Voice : (619) 822-0407
> >Flow Cytometry Core Facility FAX : (619) 822-0412
> >University of California, San Diego USA e-mail: djyoung at ucsd.edu
> > http://www-core.ucsd.edu/flow-core.html
> >*****************************************************************
> >********
> >
> >
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> >Subject: Sterilizing Sorter
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