Methanol or ethanol treatment of PBMCS

Liz Washington l.washington at vet.unimelb.EDU.AU
Sun Aug 16 23:03:02 EST 1998


Jim,
We routinely see virtually all ovine neutrophils degranulating and moving
into our mononuclear gate when they are treated with alcohol.   This occurs
when we fix & perm for internal staining either with ethanol or with the
B-D permeabilizing kit.
Liz

Elizabeth A. Washington
Senior Research Officer
Laboratory for Foetal & Neonatal Immunology
Department of Veterinary Sciences
The University of Melbourne
Parkville, VIC 3068
Australia
Phone:  61 3 9344 7349
Fax:	61 3 9344 7374
email:	l.washington at vet.unimelb.edu.au





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