Sterilizing Sorter

Dennis J. Young djyoung at UCSD.Edu
Wed Aug 12 11:52:31 EST 1998


Currently I sanitize the in line filter (and everything down stream) with
sequential 60 ml 10% acetic acid, followed by 60 ml 70% ethanol (both in
water). I let them "soak in" for 10 minutes. This is then followed by PBS.

Could I save time by using 10% acetic acid IN 70% ethanol (one solution,
one soak) and still kill every living thing in the sheath lines? 


--->    Dennis

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Dennis J. Young			Voice : (619) 822-0407
Flow Cytometry Core Facility	FAX   : (619) 822-0412
University of California, San Diego  USA	e-mail: djyoung at ucsd.edu
	http://www-core.ucsd.edu/flow-core.html
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