rhesus CD3

Carol Oxford cloxford at UCDAVIS.EDU
Fri Aug 7 16:58:33 EST 1998


Just a quick note:

We used the Biosource anti-rhesus CD3 for several months, but it was did
not recognize all rhesus macaques.  It was non-reactive in about 5-10
percent of the animals we tested.  The Pharmingen anti-human CD3 has been
reactive in all the animals I have seen.

Carol

>As far as I know, Biosource makes the only CD3 usable with these
>primates; it is an anti-monkey as anti-human does not react. Human works
>fine for anti- CD4 AND CD8 I use either Coulter / Immunotech or B-D
>(lately I use B-D) for CD4(PE), and BD for CD8 (PerCP).
>
><+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>
>
>Patricia L. Echeagaray;  echeagaray at sri.org
>Flow Cytometry Services
>Southern Research Institute - Frederick
>Pharmaceutical Research and Development  Dept.
>431 Aviation Way
>Frederick, Maryland 21701
>USA
>Voice: (301) 228-2170 or (301) 694-3232 ext 111 or 276
>   FAX: (301) 694-7223
>echeagaray at sri.org
><+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>:<+>
>
>
>>----------
>>From: 	Gillis_Otten at cc.chiron.com[SMTP:Gillis_Otten at cc.chiron.com]
>>Sent: 	Wednesday, August 05, 1998 4:39 PM
>>To: 	Cytometry Mailing List
>>Subject: 	rhesus and baboon-reactive antibodies
>>
>>
>>     Can anyone tell me what are suitable mAb for flow cytometry on rhesus
>>     and baboon lymphocytes, specifically:  CD3, CD8, and interferon-gamma
>>     (intracellular)?  Are there websites that list cross-reactive
>>     antibodies?  Thanks.
>>
>>     Gib Otten
>>     Vaccines Research
>>     Chiron Corp.
>>
>>     email to:  gillis_otten at cc.chiron.com
>>


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Carol Oxford                               _|    _|   _|_|_|_|   _|_|_|
Manager, UC Davis Optical Biology Lab     _|    _|   _|         _|    _|
University of California                 _|    _|   _|         _|    _|
Davis, California  95616                _|    _|   _|         _|    _|
(916) 752-7205                         _|    _|   _|         _|    _|
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(916) 523-0967 pager
cloxford at ucdavis.edu                 University of California, Davis
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