intracellular antigen staining (not cytokines!)

Barbara Butcher bab26 at
Thu Aug 6 09:57:02 EST 1998


i'm looking at nematode proteins that end up in epithelial cells when the
worm "nuzzles" them.  i can see these proteins very well by
confocal/epifluorescence microscopy when i stain fixed, permeabilized
monolayers.  but, when i trypsinize or edta-treat the cells to get a single
cell suspension, and try to stain in the same way (fix with 2%
formaldehyde, permeabilize with 0.075% saponin/PBS), i don't get any
signal.  can someone suggest something?  do some cells just not like being
stained in suspension?

thanks in advance.


Barbara A. Butcher, Ph.D.
James A. Baker Institute for Animal Health
Hungerford Hill Road
Cornell University
College of Veterinary Medicine
Ithaca, New York 14853

(607) 256-5647  Lab
(607) 256-5618  Office
(607) 256-5608  FAX

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