Mouse Monocyte Purification
Dennis Broud 301-827-5246 FAX 301-594-6289
BROUDD at cder.fda.gov
Tue Sep 30 12:08:29 EST 1997
You might try a product called Lympholyte-M by Cedarlane Laboratoy of Hornby,
Canada. Phone number (416)878-8891.
It's available from Accurate Chemical and Scientific Corp in the US
at phone# 1-800-645-6264.
I'm not sure who has European distribution for it.
It is Nycograde Polysucrose 400 and Sodium Diatrizoate at a density of 1.0875
plus or minus 0.0010 at 25 degrees centigrade. Ph is 6.9
NYCOGRADE is a registered trade mark of Nycomed Pharma AS of Oslo, Norwary.
You need a different density of separation media for mouse cells than you do
for human cells as the mouse cells are somewhat smaller in size and denser.
Lympholyte-M is designed for mouse lymphocytes, I don't know exactly how many
monocytes you'll get with it, but it will be better than standard
Ficoll-Hypaque, I'm sure. Give Accurate's technical service people a call
maybe they can tell you what you can expect to get.
Cedarlane also makes a Lympholyte-rat with a differnt density for separating
rat lymphocytes cells as well.
More information about the Cytometry