No subject

Ronald Rabin RRABIN at atlas.niaid.nih.gov
Fri Sep 26 06:59:20 EST 1997


The trouble with dot plots, as I understand it, is that once a
particular region is saturated with dots, there is no way one can
intuitively estimate the percentage of cells in that region.  

It seems to me (and it is quite likely that my friend and colleague Dr.
Roederer will disagree) that dotplots are OK when the region of interest
is one of extremely few events.  

I also prefer dot plots with kinetics, because fluctuations in
collection rate can obscure a response of a small population of cells
that rise above the baseline.  


ron

Ronald L. Rabin, M.D.
NIAID/NIH
Building 10/Room 11N228
10 Center Drive MSC 1888
Bethesda, MD   20892-1888
Phone:  (301) 402-4910
FAX:      (301) 496-7383



Flow-ers,

Mario Roederer said:



> Of course, you will agree that dot plots are completely inappropriate.
> (Everyone:  please stop publishing data with single-color dot plots!)

You've lost me on this one.  Being a relative new-comer to flow 
cytometry, I don't see what is wrong with dot plots.  Please educate 
me.  If dot plots are "completely inappropriate", is there ever a 
situation when they would be appropriate to use?

Thanks,
Ken Schafer
    kschafer at lucy.tli.org
    Kenneth A Schafer
    Lovelace Respiratory Research Institute
    PO Box 5890
    Albuquerque, NM  87185
    505-845-1126
    505-845-1198 (fax)                        

                      




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