FACS 440 assistance: update.
Raymond B. Hester
rhester at jaguar1.usouthal.edu
Fri Sep 12 15:16:20 EST 1997
Our FACS 440 will not display dots or accumulating histograms on the data
display screen although pulses appear on the console pulse display (the
alpha numerics do appear, however, on the console data display).
We started swapping cards and boards from a used 440 we bought for this
purpose and replacement of the DMG board in the ND624 restored the dots -
for a time. The problem surfaced again the following day!
One other piece of information - our software runs on a VAX and on the
VAX monitor one can see the dots. We could perhaps operate in this
fashion except for the fact that it makes alignment almost impossible,
it's difficult to see the dots representing polystyrene beads on this
screen, and they don't seem to respond as quickly (no matter how you set
it up timewise) to adjustments in the stream or fluorescence pickup lens
as did the dots on the FACS console data display.
Does this give anyone a clue as to what has gone wrong?
Thanks again for any help.
Raymond B. Hester, Ph.D. Someday, after we have mastered the
Flow Cytometry Lab winds, the waves, the tides and
CSAB 357, Biotechnical Services gravity, we shall harness the
The University of South Alabama energies of love. Then, for the
Mobile, AL 36688-0002 second time in the history of
Email: rhester at jaguar1.usouthal.edu the world, man will have
Voice: (334) 460-6029 discovered fire.
FAX: (334) 460-6073 -Teilhard de Chardin
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