Yeast DNA analysis, and sadistics thanks!

Steve G. Hilliard steve at habanero.cb.uga.edu
Fri Nov 21 17:25:20 EST 1997


First a "thank you" to all of you who set me straight regarding the
intimidating K-S test.  Several mentioned that B-D software provides 
this test--do any other packages offer it?  I'll have to check it
out, even though that student didn't seem interested in wading in that
deeply.

Now, to David,
You certainly have my sympathies.  I had a user who was doing the same
sort of experiments a few years ago, and we never produced any data I
believed in.  We saw exactly the same sort of variation, but the client
never understood that this was a problem.  In fact they wanted me to
change the HV for each sample so that they lined up properly!  Sometimes
ya just wanna smack em....

Do you have a copy of Methods in Cell Biology v42, PartB ?  The chapter
on yeast by Dien, Peterson and Srienc has some very clear protocols,
and the data presented look much nicer than anything we got.  I never
got a clear idea of how my client prepared their samples, but if I'm ever
faced with yeast again I'll make them start with this chapter.  I'll be 
happy to send you a copy if you (or your library) doesn't have the book.

Steve
---------------------------------------------------------------
Steve G. Hilliard        steve at habanero.cb.uga.edu        
University of Georgia Cell Analysis Facility 
http://floweb.cb.uga.edu/Floweb/
---------------------------------------------------------------


On Thu, 20 Nov 1997, David McFarland wrote:

> 
> I have a client that is staining yeast with PI and then doing cell cycle
> analysis.  From what I understand, the wild type yeast normally spend most of

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