apoptosis

Mayumi Naramura MNARAMURA at atlas.niaid.nih.gov
Sun Nov 2 13:20:33 EST 1997


I'm trying to study apoptosis of
1. fresh mouse thymocytes induced by
2. TCR stimulation using
3. flow cytometry, either by 
	3a. Annexin V or by 
	3b. TUNEL. 
I know there are tremendous amount of literature related to this, but so
far I have not come across with ones that contain all the "keywords" I
listed above. Specifically, I found most of the study has been done
using "immature" T cell lines or hybridomas instead of "fresh mouse
thymocytes". 
I tried it a couple of times, but I cannot see any difference between
cells stimulated with anti-CD3 and non stimulated control because a
significant portion of cells are Annexin V (+) in the control. Any
suggestions?   
Mayumi Naramura, M.D.
NIH/NIAID
Lab of Immunology
Twinbrook II, Rm. 125
12441 Parklawn Drive
Rockville, MD 20852
Phone:  301-402-4595
FAX:      301-594-2522
e-mail:mnaramura at atlas.niaid.nih.gov



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