CD14 negative monocytes

RICHARD L. DARLEY Darley at cardiff.ac.uk
Tue Mar 25 11:02:20 EST 1997


I have been carrying out culture of CD34+ cells in the presence of
IL-3, GM- & G-CSF and following myeloid differentiation using cell
surface markers.

I am finding that staining with CD14 is grossly under-estimating the 
monocytic content of these cultures as judged by morphology.

One of my colleagues has made similar observations.  Additionally he 
found that CD14+ AML cells lost expression of this marker when in 
culture.

Has anyone had similar experiences or any good suggestions for
alternative markers which more reliably indicate monocytic
differentiation under these conditions?


################################################################

 From: Dr Richard Darley, Ph.D.
LRF Differentiation Unit
Department of Haematology (A7)
University of Wales College of Medicine
Heath Park
Cardiff, CF4 4XN
U.K.
tel  (+44)  12 22 74 34 85
or (+44)  12 22 74 45 24
Fax (+44)  12 22 74 45 23
e-mail: darley at cf.ac.uk



More information about the Cytometry mailing list