CPRUSSIN at atlas.niaid.nih.gov
Thu Jun 5 09:26:53 EST 1997
Do you need to use a surface marker? How about using one of the
membrane stable PKH dyes sold by Sigma? These are incredibly bright,
don't "bleed" from one cell to the next and are functionally inert.
You would just incubate your Jurkats in the dye prior to your adhesion
From: kjartan.egeberg at medisin.ntnu.no
Sent: Wednesday, June 4, 1997 3:46 AM
Subject: Jurkat marker
Yesterday I sent a request for a good surface-marker to stain Jurkat
Rob McGilp wrote that CD95 is well expressed on these cells. Thank you
your reply. It is true that Jurkat express CD95, but since Fas/Fas-L
part of the study (adhetion of apoptotic cells), I have to use
marker to follow the Jurkats. I intend to use cell tracing. But I need
surface-marker to separate those adhered to targets from those that
I'm still on my tip-toes with hopes for a good surface-marker.
Inst. of Canser Research and Mol. Biol.
NTNU, Trondheim, Norway
E.mail: kjartan.egeberg at medisin.ntnu.no
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