Simple counts with flow: chicken rbc's

Irene J. Check, Ph.D. ijc059 at lulu.acns.nwu.edu
Thu Feb 27 18:02:36 EST 1997


In a manner analogous to spiking the sample with a known concentration of
microspheres according to the recipe posted by Chris Groves, you could spike
the sample with a known concentration of chicken erythrocytes.  This concept
was published by MR Loken and VL Sheckler (Transfusion 33:256-61, 1993) as
part of a method for detecting low concentrations of WBC in platelet products.

A commercial alternative is to use Coulter's FlowCount microspheres
(concentration/mcl is already measured.  They're expensive, though.
>
Irene J. Check, Ph.D.
Director, Clinical Immunology, Flow Cytometry, and Cytogenetics
Evanston Hospital
2650 Ridge Ave
Evanston, IL 60201

847-570-2739 (voice)
847-570-1938 (fax)




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