PHA aggregation (fwd)

mcfarland david c mcfarlnd at ux1.cso.uiuc.edu
Thu Feb 27 10:01:10 EST 1997


I had the same problem.  PHA by definition is a hemagglutinin.  I started 
stimulating with SEB instead.

David McFarland
University of Illinois Biotechnology Center
Flow Cytometry Facility

---------- Forwarded message ----------
Date: Wed, 26 Feb 1997 13:35:59 +0000
From: tlr at mrc-lmb.cam.ac.uk
To: cyto-inbox
Subject: PHA aggregation


Dear colleagues,

I am looking at intracellular cytokines produced by CD14+/CD3+ cells from
PBMC cultures, in response to PHA stimulation. I am having problems with
cell aggregation . Has anyone used a similar system and how did they get
round it? Stimulating with PMA/ionophore is not an option at the present
time.

Thank you in advance,

Tom Rowland






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