PHA aggregation

tlr@mrc-lmb.cam.ac.uk tlr at mrc-lmb.cam.ac.uk
Wed Feb 26 08:35:59 EST 1997


Dear colleagues,

I am looking at intracellular cytokines produced by CD14+/CD3+ cells from
PBMC cultures, in response to PHA stimulation. I am having problems with
cell aggregation . Has anyone used a similar system and how did they get
round it? Stimulating with PMA/ionophore is not an option at the present
time.

Thank you in advance,

Tom Rowland





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