GFP

David L. Haviland, Ph.D. dhavilan at imm2.imm.uth.tmc.edu
Tue Feb 25 11:29:37 EST 1997


Hi:

I was curious if people had been successfully analysed GFP levels within
transfected cells using flow?
I once had an investigator try something like this (though I do *not* know
if it was GFP, per se) and the experiment was a complete bust!  There was
something about the excitation/emmision that was not near instantaneous and
there was enough delay that no fluoresence could be detected except when
using a flourescent microscope.

David




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