Clean machines

Dr. Huw S. 'Zip' Kruger Gray. ZIP at flowcyt.cyto.purdue.edu
Mon Feb 24 17:35:05 EST 1997


My penny's worth:- 

    Ethanol also is a most effective fixative agent (!) & as such will 
fix any residual protein, et. al. on to the walls of your instrument's 
tubing, flow cell, etc. very efficiently as well! 

"Zip". 
>>>---> 

----------------------------------------------------------------------

> Date sent:      Mon, 24 Feb 1997 11:44:40 +0000
> To:             Cytometry Mailing List <cytometry at flowcyt.cyto.purdue.edu>
> From:           Arnold Pizzey <a.pizzey at ucl.ac.uk>
> Subject:        Clean machines

> 
> 
> Greetings all.
> 
> 
>         I would question the efficacy of using 70% ETOH alone as a
> sterilization agent specifically in flow as well as generally in the
> laboratory. Although ETOH has some bactericidal activity against common
> water-borne contaminants such as the pseudomonads and the like I would
> imagine that most airborne contaminants that float into the sheath tank
> would be of the Gram-positive persuasion and as such quite resistant to the
> effect of ETOH.
>     As for swabbing down  external surfaces with 70% ETOH, I would regard this
> as an excellent  strategy for mobilizing bacterial aerosol.
> 
> On the few occasions when I have had to carry out sterile sorting, my
> strategy is as follows:
> 
>     Clean sort nozzle/flow cell /sheath tank with a proprietary detergent and
> rinse in sterile H2O.
>     Wash the sort collection area down with a proprietary detergent
>     Bypass the in-line sheath filter, Then flush system with following:
>     1)H2O
>     2)Surface active agent e.g. 0.1% DECON 90
>     3)Sterile H20
>     4)Sterile sheath fluid
>     5)Fit new sterile in-line sheath filter.
> 
> Regards .
> 
>                     Arnold.
> _____________________________________________________
> Arnold Pizzey
> Department of Haematology
> 98 Chenies Mews
> London WC1E 6HX
> E-mail  a.pizzey at ucl.ac.uk.  
> Voice   (0)171 209 6234; 
> fax     (0)171 209 6222.
> _____________________________________________________
> 



More information about the Cytometry mailing list