Sheath Solution

David Chambers davidc at flosun.salk.edu
Mon Feb 24 12:42:09 EST 1997


I couldn't resist defending my favorite sheath fluid, after reading
Vivian's comments.

I've been using it for 18 months.  Only once did the boxes turn up damaged
- and they obviously had been beaten up by the shipper.  Riese replaced
them immediately.  I have not seen leaky containers for over a year now;
perhaps Vivian's shippers are overzealous!  This reminds me of a problem we
had in London with cracked tissue culture tubes - 20% of the box would turn
up cracked.  After some investigation it turned out that the shipping company's
employees were jumping up and down on the boxes in order to force them into
a full van..... :-)

Apart from the regular sheath concentrate, I also use their preservative-free
concentrate for sorting, and have had no problems whatsoever with it.  It comes 
in nice strong 1 litre bottles.

--David.


On 23-Feb-97 Vivian Wu wrote:
>
>Hi, All,
>
>I've had several replies regarding sheath solution from Biosure. The
>response is as follows:
>1. Cheaper than FACSflow and Hemaline (and I'll add that it is competitive
>pricing with Isoton by Coulter)
>2. It is shipped in manageable sizes (avoids paying to send all that
>water across the continent. I'll add that the 20 L cubes are heavy and has
>managed to put my back out a few years ago).
>3. A spigot comes with each container (and they fit with no leaks!)
>4. The containers and boxes can be recycled for other uses.
>5. Works very well although there are reservations when it comes to
>sorting due to their preservatives.
>
>I'll agree to the above except #4. We have been using it for about 6
>months. We have found their containers to be FAULTY! Our shipments from
>Biosure almost always have leakages. Boxes are WET upon arrival! Crusting
>from NaCl around the lid appears often! The two dents at the opposite end,
>where the plastic halves join are their weakest spot. After reconstiution
>with dH2O, we now press that end of the container and will OFTEN find a
>gaping hole where it will actually squirt out. Could it be damage due to
>shipping to the far west? I was surprised that nobody mentioned the
>container's defects! Is it only us that is experiencing this?
>
>Vivian Wu (e-mail: ffwu at unixg.ubc.ca)
>Immunology Lab
>Vancouver Hospital & Health Sciences Centre

----------------------------------
E-Mail: David Chambers <davidc at flosun.salk.edu>
Date: 24-Feb-97
Time: 09:42:09

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