BD fastimmune system
Michelle N Fiordalisi
mnfiord at med.unc.edu
Fri Feb 21 08:44:21 EST 1997
I have been working with BDs FastImmune anti-IFNg and anti-IL-4
antibodies for about 2 months (but only with normal controls) and have
been very pleased with the results. I have been following BD's protocol
and have had success with each experiment. I have used both the
IFNg/IL-4 combo with the isotype combo and individual reagents with
success. Although BD sells a special formulation of IgG1 PE, I have been
titering their standard formulation and think it will work nearly as well
as a control for IL-4 PE.
I have not tried using saponin for permeablization but will be comparing
BD's FACSPerm to Caltag's Fix N' Perm.
If you would like more details, please email me directly.
Michelle N. Fiordalisi, Ph.D.
Clinical Immunology Fellow
University of North Carolina Hospitals
email: mnfiord at med.unc.edu
Phone: 919-966-4058 FAX: 919-966-0486
On Thu, 20 Feb 1997, Vishal Gulati wrote:
> Hi! Has anyone on the list used BD Fastimmune Intracellular cytokine
> staining reagents. I have been using 'conventional' indirectly lablled
> cytokine antibodies and am planning to switch over.
> Also, has someone compared saponin with BD/any other commercial
> permeablising reagent .
> Id be grateful if those of you out here in the know of things share their
> Thanks in advance
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