j.nelson at auckland.ac.nz
Tue Feb 18 16:48:55 EST 1997
We want to test for Cyclin D1 on peripheral blood and FNA samples from
patients with possible B lymphoproliferative disorders.
I have bought some Immunotech Cyclin D1 (Cat No 1740) and tried the method
we use for Ki-67 and cytoplasmic CD3 and CD22: Acetone fix of cytospins
followed by standard 2 stage immunofluorescence. We are testing the
antibody at 1/10 and 1/100 to start with.
We haven't seen any convincing positivity although we have had several
possible Mantle cell lymphomas.
Also what sort of staining is seen - is it similar to TdT or Ki-67?
While we wait for a positive to turn up perhaps someone who has had
experience with this antibody in the context of B LPD could advise if the
method is inappropriate.Should we try flow?
Department of Molecular Medicine
The University of Auckland
Phone: (+64)(9) 373-7599 ext 6381
Fax: (+64)(9) 373-7292
More information about the Cytometry