A clean machine -Reply
t-delohery at ski.mskcc.org
Mon Feb 17 01:32:24 EST 1997
Seems like we're the only ones that use detergent. We fill the tubing
with a 60cc syringe with 1:100 7X detergent in millipore H2O and let it
sit for an hour or two. This is followed by a millipore H2O rinse, then
60cc of 70% EtOH to help flush the detergent, followed by half a liter of
PBS. The 7X and EtOH is filtered with a 0.45 micron syringe filter.
Detergent is never used in the sheath tank or filter housing. We do this
about once per month and it works beautifully.
The only time I use bleach is if there's a known contaminant and we haven't
had one of those in many years. Bleach does some pretty horrible things to
the tubing not to mention your nozzle tip.
Thomas Delohery | Internet: t-delohery at ski.mskcc.org
Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729
Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019
1275 York Ave. Box 98 |
New York, NY 10021 |
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