ulrik.sprogoe-jakobsen at OUH.DK
Fri Feb 14 18:59:09 EST 1997
Michelle Miller wrote:
>To FACSort/FACScan users,
>We recently upgraded our FACSort computer from an HP system to a PowerMac
> In general we have been happy with this, although CellQuest could still has
>some way to go to take advantage of all Macs have to offer (but is better than
>Lysis II in most ways). However, we are having a sporadic, but persistent and
>annoying problem - when attempting to connect to the cytometer from
> program we often get a message saying that the cytometer cannot be found.
>This is despite turning the FACS on before the computer, etc. This can go on
>for hours before it decides that the FACS really is there and lets us
> for aquisition. BD suggested reloading the software, which had no
> did rebuilding the desktop. Our computer consultant has suggested that
>fragmentation of the hard disk is to blame, and that the recent high humidity
>in Sydney may also be implicated.
>Has anyone else had this problem with their Mac/FACS interface? We use Macs
>exclusively in our institute (with the exception of the old HP) and the
> networked but the harddisk cannot be accessed by other computers without
> checking that the FACSort is not in use. Any suggestions would be very
>Thanks in advance,
>Johnson and Johnson Research Labs
>email: jjphar13 at angis.su.oz.au
Like for any other problem with Mac software/hardware I would try the
following in the order mentioned:
1. Rebuild the desktop (use a utility like TechTool 1.1.2 (freeware) by
MicroMat, instead of the MacOS build-in option, as this will delete the old
desktop file, instead of rebuilding the existing one)
2. Zap the parameter RAM (again use TechTool).
3. Restart with extensions off (except the inits/cdevs neccesary for
CELLQuest to run)
4. Reinstall BD software (remember to trash BD inits/cdevs and preferences) .
5. Run a harddisk check program (better Norton Utilities, than Apples Disk
First Aid) from a floppy disk.
6. Low level reformat the boot disk (internal harddisk), update the
harddisk driver and reinstall system software and BD software.
Dept. Clinical Immunology
Odense University Hospital
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