A clean machine -Reply

Flow Cytometry Mail Group FLOWUSER at cyto.medsch.ucla.edu
Fri Feb 14 17:01:00 EST 1997



Here is to another unusual cleaning solution: we have started to run 20%   
acetic acid through the lines on our sorters as a cleaning and   
disinfecting? agent to replace bleach and ethanol which seemed to   
interfer with functional activity of sorted cells.  We always follow with   
distilled water and it is easy to check that the acid is rinsed out by   
using a tube with media that contains phenol red to collect the fluid   
from the nozzle.  We have seen no ill effects on the tubing.
Ingrid

 ----------
From:   
 owner-cyto-sendout[SMTP:owner-cyto-sendout at flowcyt.cyto.purdue.edu]
Sent:  Tuesday, February 11, 1997 5:22 PM
To:  Cytometry Mailing List
Subject:  Re: A clean machine -Reply


        Reply to:   RE>>A clean machine -Reply
We've been using a dilute sodium hydroxide solution to clean the tubing   
in
our three
FACscans for several years now.  We used to use bleach, as recommended by   
the
manufacturer, and sometimes we would have clogs which never seemed to   
clear up
completely.  Our protein chemist recommended NaOH to me late one   
afternoon
when
I was particularly frustrated with a stubborn clog.  The NaOH cleared it   
right
out, and from then on we've used it regularly to keep the lines clear   
with few
problems.  I've always wondered if anyone else has tried it and how it   
has
worked for them.
Florence

 --------------------------------------
Date: 2/11/97 4:21 PM
To: cyto-inbox
From: Jim Zanghi

>Small beads staying in the instrument can be a problem.  Triton X-100 at
>0.1% (the same concentration used to permeabilize cells) in water will
>remove most of them.
>
>Betsy Robertson
>>
>> Running ethanol
>> after the beads seems to help push them through more quickly.
>>
>> Tony Bakke

I was wondering about what types of solutions can be run through a flow
cytometer without causing damage or corrosion.   Is pure ethanol okay?   
  On
the advise of others, I  have run 50% hot bleach to clear up clogs, but I
was very weary about doing this since bleach corrodes stainless steel.
I've always followed with a 5 minute water rinse, but this concentration   
of
bleach seems a bit extreme (we routinely use 10% bleach at room temp).
If 0.1% triton or 70% ethanol is as effective as 50% hot bleach, than   
this
seems to be the way to go.   Any thoughts about this?  What do
manufacturers recommend?

Jim

 --
James A. Zanghi
Dept. of Chemical Engineering
Northwestern University
Evanston, IL



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From: zanghi at merle.acns.nwu.edu (Jim Zanghi)
Subject: Re: A clean machine -Reply







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