WARNING: Not strictly serious business, may contain humor

Sue DeMaggio suedemag at e4e.oac.uci.edu
Fri Feb 14 10:55:14 EST 1997

I love the fact that these two messages appeared within minutes of each
other on my computer and have exactly the opposite suggestions!  Ain't flow
fun?  By the way, I'm glad there are people with a sense of humor in this
field - it definitely breaks the tension and monotony of reading one email
after the other.  I'm putting a warning in the subject line so those who
don't appreciate cat scans and cytomutts are forewarned!  The rest of you,
enjoy the chuckle!
Sue DeMaggio

Derek Davies suggests:

Hi Michelle,

This connectivity problem seems to be common, although I for one have yet
to see a definitive explanation or universal workround.

I have been told that the problem stems from the architecture of one of
the bus boards - unfortunately as I don't speak fluent jargon, this means
little. Apparently BD and Apple are working on something that will cure it
but that may be some way down the line (This is UK info, perhaps the US
end know more?)

>From a practical day to day point of view, I always make sure that the
FACS is switched on first and left for at least 5 minutes before the
computer is switched on (an arbitrary time determined by the time taken
to get a coffee). We have a FACSCalibur connected to a 7600. If for any
reason this fails, I always switch both off and wait a minute or two
before trying again.

At 01:47 PM 2/13/97 +-1100, Peter Chapple wrote:
>We also have communications problems between the PowerMAC and cytometer -
in our case the cytometer is a FACSCalibur, but I suspect a similar/same
mechanism may be involved.
>At the outset I must say that I have no proof of what I'm about to say ...
only an observation I have made over many months.
>It seems to me that the timing of when cytometer and computer are
switched-on is very important in establishing the dialogue between the two.
Obviously you power-up the cytometer first, but then the computer must
follow within the next 1-4minutes (approx). If you do this then there is
rarely a problem - if the cytometer has been powered-up for longer than
4ish minutes before the computer communications are unlikely to work!.
> It follows from this that if you are operating the instrument and the
computers has one of those dreaded *error type 11* situations which obliges
you to do a re-start on the computer - then it's necessary to power-down
the cytometer as well and start from scratch :-(
>These observations are true for the FACSCalibur - I'm presuming the same
interface board resides in your FACSort
>Hope this is useful 
>Peter Chapple
>Peter MacCallum Cancer Institute
>Melbourne AUSTRALIA
>>We recently upgraded our FACSort computer from an HP system to a PowerMac
>>In general we have been happy with this, although CellQuest could still has
>>some way to go to take advantage of all Macs have to offer (but is better
>>Lysis II in most ways).  However, we are having a sporadic, but
persistent and
>>annoying problem - when attempting to connect to the cytometer from
Writing the
>>program we often get a message saying that the cytometer cannot be found.
>>This is despite turning the FACS on before the computer, etc.  This can
go on
>>for hours before it decides that the FACS really is there and lets us
access it
>>for acquisition.  BD suggested reloading the software, which had no
effect, nor
>>did rebuilding the desktop.  Our computer consultant has suggested that
>>fragmentation of the hard disk is to blame, and that the recent high
>>in Sydney may also be implicated.
>>Has anyone else had this problem with their Mac/FACS interface?  We use Macs
>>exclusively in our institute (with the exception of the old HP) and the
7600 is
>>networked but the harddisk cannot be accessed by other computers without
>>checking that the FACSort is not in use.  Any suggestions would be very
>>Thanks in advance,
>>Michelle Miller
>>Johnson and Johnson Research Labs
>>Sydney, Australia
>>email: jjphar13 at angis.su.oz.au

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